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Methods Mol Biol. 2017;1497:47-55.

Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research.

Author information

1
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.
2
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria. wolfgang.busch@gmi.oeaw.ac.at.

Abstract

Genome-wide association (GWA) mapping is a powerful method for the identification of alleles that underlie quantitative traits. It enables one to understand how genetic variation translates into phenotypic variation. In particular, plant hormone signaling pathways play a key role in shaping phenotypes. This chapter presents a protocol for genome-wide association mapping of root traits of Arabidopsis thaliana in the context of hormone research. We describe a specific protocol for acquiring primary and lateral root trait data that is appropriate for GWA studies using FIJI (ImageJ), and subsequent GWA mapping using a user-friendly Internet application.

KEYWORDS:

GWAS; Hormones; Natural variation; Phenotyping; Root development

PMID:
27864757
DOI:
10.1007/978-1-4939-6469-7_6
[Indexed for MEDLINE]

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