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Science. 2016 Nov 4;354(6312). pii: aag1550.

A transcription factor hierarchy defines an environmental stress response network.

Author information

1
Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
2
Computational Biology Department, School of Computer Science, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
3
Genomic Analysis Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
4
Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA. ecker@salk.edu.
5
Howard Hughes Medical Institute (HHMI), Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

Abstract

Environmental stresses are universally encountered by microbes, plants, and animals. Yet systematic studies of stress-responsive transcription factor (TF) networks in multicellular organisms have been limited. The phytohormone abscisic acid (ABA) influences the expression of thousands of genes, allowing us to characterize complex stress-responsive regulatory networks. Using chromatin immunoprecipitation sequencing, we identified genome-wide targets of 21 ABA-related TFs to construct a comprehensive regulatory network in Arabidopsis thaliana Determinants of dynamic TF binding and a hierarchy among TFs were defined, illuminating the relationship between differential gene expression patterns and ABA pathway feedback regulation. By extrapolating regulatory characteristics of observed canonical ABA pathway components, we identified a new family of transcriptional regulators modulating ABA and salt responsiveness and demonstrated their utility to modulate plant resilience to osmotic stress.

PMID:
27811239
PMCID:
PMC5217750
DOI:
10.1126/science.aag1550
[Indexed for MEDLINE]
Free PMC Article

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