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Stem Cell Res. 2016 Nov;17(3):544-546. doi: 10.1016/j.scr.2016.09.023. Epub 2016 Sep 23.

Episomal plasmid-based generation of an iPSC line from a 79-year-old individual carrying the APOE4/4 genotype: i11001.

Author information

1
Institute for Pharmacology und Clinical Pharmacy, Faculty of Pharmacy, Philipps University Marburg, Karl-von-Frisch-Str. 1, 35034 Marburg, Germany.
2
Centre for Human Genetics, Faculty of Medicine, Philipps University Marburg, Baldingerstra├če, 35033 Marburg, Germany.
3
Institute for Pharmacology und Clinical Pharmacy, Faculty of Pharmacy, Philipps University Marburg, Karl-von-Frisch-Str. 1, 35034 Marburg, Germany. Electronic address: nieweg@staff.uni-marburg.de.

Abstract

In this study, lymphoblastoid cells derived from a 79-year old individual with a history of progressive presenile dementia, were used to generate iPS cells, employing episomal plasmids expressing OCT4, SOX2, KLF4, LIN28, L-MYC and a p53 shRNA. The individual was homozygous for the APOE4 allele. The resulting iPS cells had a normal karyotype, retained the APOE4/4 genotype, expressed pluripotency markers, were free of genomically integrated plasmids, and could be differentiated into cell type representatives from the three germ layers in vitro.

PMID:
27789405
DOI:
10.1016/j.scr.2016.09.023
[Indexed for MEDLINE]
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