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Methods Mol Biol. 2017;1493:237-246.

Performing Axon Orientation Assays with Secreted Semaphorins and Other Guidance Cues.

Author information

1
University of Lyon, University Claude Bernard Lyon 1, NeuroMyoGene Insitute (INMG) CNRS UMR5310-INSERM U1217, 16 rue Raphaël Dubois, Lyon 69000, France.
2
University of Lyon, University Claude Bernard Lyon 1, NeuroMyoGene Insitute (INMG) CNRS UMR5310-INSERM U1217, 16 rue Raphaël Dubois, Lyon 69000, France. valerie.castellani@univ-lyon1.fr.

Abstract

The guidance of axons within the developing nervous system is orchestrated by a variety of cues that successively and complementary attract or repel axons to achieve a stereotyped wiring of neural circuits. Here we present a version of a method that has been widely used to identify and characterize the effect of guidance cues on the orientation of axons. We describe the coculture, within a three-dimensional environment, of dorsal spinal cord explants together with a cell aggregate secreting a candidate cue and the method to quantify the effect of this cue on axon orientation.

KEYWORDS:

Axon guidance; Cell aggregate; Coculture; Plasma clot; Spinal cord explants

PMID:
27787855
DOI:
10.1007/978-1-4939-6448-2_17
[Indexed for MEDLINE]

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