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Sci Rep. 2016 Oct 25;6:35813. doi: 10.1038/srep35813.

A role for intestinal TLR4-driven inflammatory response during activity-based anorexia.

Belmonte L1,2,3,4, Achamrah N1,2,3,4, Nobis S1,2,3, Guérin C1,2,3, Riou G1,3,5, Bôle-Feysot C1,2,3, Boyer O1,3,5,6, Richard V1,3,7, Rego JC1,3,8, Déchelotte P1,2,3,4, Goichon A1,2,3, Coëffier M1,2,3,4.

Author information

1
Normandie Univ, UR, Rouen, France.
2
INSERM unit 1073, Rouen, France.
3
Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.
4
Rouen University Hospital, Nutrition Department, Rouen, France.
5
Flow cytometry facility CyFlow, Rouen, France.
6
INSERM, U905, Rouen, France.
7
INSERM, U1096, Rouen, France.
8
Animal Behavior Platform SCAC, Rouen, France.

Abstract

Anorexia nervosa (AN) is associated with low-grade systemic inflammation and altered gut microbiota. However, the molecular origin of the inflammation remains unknown. Toll-like receptors are key regulators of innate immune response and their activation seems also to be involved in the control of food intake. We used activity-based anorexia (ABA) model to investigate the role of TLR4 and its contribution in anorexia-associated low-grade inflammation. Here, we found that ABA affected early the intestinal inflammatory status and the hypothalamic response. Indeed, TLR4 was upregulated both on colonic epithelial cells and intestinal macrophages, leading to elevated downstream mucosal cytokine production. These mucosal changes occurred earlier than hypothalamic changes driving to increased levels of IL-1β and IL-1R1 as well as increased levels of plasma corticosterone. Paradoxically, TLR4-deficient mice exhibited greater vulnerability to ABA with increased mortality rate, suggesting a major contribution of TLR4-mediated responses during ABA-induced weight loss.

PMID:
27779218
PMCID:
PMC5078809
DOI:
10.1038/srep35813
[Indexed for MEDLINE]
Free PMC Article

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