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J Microbiol Methods. 2017 Jan;132:95-98. doi: 10.1016/j.mimet.2016.10.004. Epub 2016 Oct 8.

Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007.

Author information

1
College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China.
2
College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China. Electronic address: jrye@njfu.edu.cn.
3
College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China; The Connecticut Agricultural Experiment Station, Valley Laboratory, 153 Cook Hill Road, Windsor, CT, United States.

Abstract

Burkholderia pyrrocinia strain JK-SH007 isolated from poplar stems plays a highly significant role in the growth promotion and the biocontrol of poplar canker during colonization in poplar. In this research, the ideal reference gene was filtered and determined for the transcript normalization. Additionally, the expression of pyrG under all four conditions was relatively stable in B. pyrrocinia JK-SH007.

KEYWORDS:

Burkholderia pyrrocinia; Expression stability; RT-qPCR; Reference genes

PMID:
27725176
DOI:
10.1016/j.mimet.2016.10.004
[Indexed for MEDLINE]

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