Format

Send to

Choose Destination
Nat Commun. 2016 Oct 3;7:13018. doi: 10.1038/ncomms13018.

EIN2-dependent regulation of acetylation of histone H3K14 and non-canonical histone H3K23 in ethylene signalling.

Zhang F1,2, Qi B1,2, Wang L1,2, Zhao B2, Rode S2, Riggan ND2, Ecker JR3,4, Qiao H1,2,5.

Author information

1
Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, Austin, Texas 78712, USA.
2
Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.
3
Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 USA.
4
Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, California 92037 USA.
5
Department of Molecular Biosciences, The Center for Systems and Synthetic Biology, Austin, Texas 78712, USA.

Abstract

Ethylene gas is essential for many developmental processes and stress responses in plants. EIN2 plays a key role in ethylene signalling but its function remains enigmatic. Here, we show that ethylene specifically elevates acetylation of histone H3K14 and the non-canonical acetylation of H3K23 in etiolated seedlings. The up-regulation of these two histone marks positively correlates with ethylene-regulated transcription activation, and the elevation requires EIN2. Both EIN2 and EIN3 interact with a SANT domain protein named EIN2 nuclear associated protein 1 (ENAP1), overexpression of which results in elevation of histone acetylation and enhanced ethylene-inducible gene expression in an EIN2-dependent manner. On the basis of these findings we propose a model where, in the presence of ethylene, the EIN2 C terminus contributes to downstream signalling via the elevation of acetylation at H3K14 and H3K23. ENAP1 may potentially mediate ethylene-induced histone acetylation via its interactions with EIN2 C terminus.

PMID:
27694846
PMCID:
PMC5063967
DOI:
10.1038/ncomms13018
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center