Format

Send to

Choose Destination
J Cell Biol. 2016 Sep 26;214(7):875-89. doi: 10.1083/jcb.201603086. Epub 2016 Sep 19.

Transition zone assembly and its contribution to axoneme formation in Drosophila male germ cells.

Author information

1
Université Claude Bernard Lyon 1, Institut National de la Santé et de la Recherche Médicale U1217, Institut NeuroMyoGène, Centre National de la Recherche Scientifique UMR 5310, F-69100 Lyon, France.
2
Université Claude Bernard Lyon 1, Institut National de la Santé et de la Recherche Médicale U1217, Institut NeuroMyoGène, Centre National de la Recherche Scientifique UMR 5310, F-69100 Lyon, France durand-b@univ-lyon1.fr.

Abstract

The ciliary transition zone (TZ) is a complex structure found at the cilia base. Defects in TZ assembly are associated with human ciliopathies. In most eukaryotes, three protein complexes (CEP290, NPHP, and MKS) cooperate to build the TZ. We show that in Drosophila melanogaster, mild TZ defects are observed in the absence of MKS components. In contrast, Cby and Azi1 cooperate to build the TZ by acting upstream of Cep290 and MKS components. Without Cby and Azi1, centrioles fail to form the TZ, precluding sensory cilia assembly, and no ciliary membrane cap associated with sperm ciliogenesis is made. This ciliary cap is critical to recruit the tubulin-depolymerizing kinesin Klp59D, required for regulation of axonemal growth. Our results show that Drosophila TZ assembly in sensory neurons and male germ cells involves cooperative actions of Cby and Dila. They further reveal that temporal control of membrane cap assembly by TZ components and microtubule elongation by kinesin-13 is required for axoneme formation in male germ cells.

PMID:
27646273
PMCID:
PMC5037411
DOI:
10.1083/jcb.201603086
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center