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Tumour Biol. 2016 Nov;37(11):14553-14563. Epub 2016 Sep 8.

LINC00312 inhibits the migration and invasion of bladder cancer cells by targeting miR-197-3p.

Author information

1
Department of Urology, Huai'an First People's Hospital affiliated with Nanjing Medical University, No. 6 Beijing West Road, 223300, Huai'an, Jiangsu Province, People's Republic of China.
2
Department of Urology, Huai'an Hospital affiliated of Xuzhou Medical College, Huai'an, 223002, People's Republic of China.
3
Department of Urology, Huai'an First People's Hospital affiliated with Nanjing Medical University, No. 6 Beijing West Road, 223300, Huai'an, Jiangsu Province, People's Republic of China. Meng_31junsong@163.com.

Abstract

To investigate the influence of the long non-coding RNA LINC00312 on bladder cancer (BC) cell invasion and metastasis by targeting miR-197-3p. BC and corresponding adjacent tissues were collected. LINC00312 and miR-197-3p were measured, and their correlation was detected through quantitative real-time PCR (qRT-PCR). BC cell line T24 was transfected and grouped (five groups) according to different transfection conditions. A scratch test was applied to analyze cell migration, and a Transwell assay was used to test cell invasion ability. Western blotting was to measure matrix metalloproteinase (MMP)-2, MMP-9, and the tissue inhibitor of metalloproteinase 2 (TIMP2) protein levels. qRT-PCR indicated that LINC00312 expression was lower but miR-197-3p expression was higher in BC tissues compared with adjacent tissues; LINC00312 was negatively correlated with miR-197-3p. The migration test revealed that the downregulation of miR-197-3p and overexpression of LINC00312 inhibited cell migration and invasion abilities, while the overexpression of miR-197-3p and the upregulation of LINC00312 promoted cell migration and invasion. BC cells with downregulated miR-197-3p or upregulated LINC00312 had low MMP-2 and MMP-9 levels but high TIMP2. LINC00312 inhibited BC cell invasion and metastasis through mediating miR-197-3p.

KEYWORDS:

Bladder cancer; Cell invasion; Cell migration; LINC00312; Long non-coding RNA; miR-197-3p

PMID:
27631965
DOI:
10.1007/s13277-016-5303-8
[Indexed for MEDLINE]

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