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Antimicrob Agents Chemother. 2016 Oct 21;60(11):6973-6976. doi: 10.1128/AAC.01457-16. Print 2016 Nov.

A Model for Transposition of the Colistin Resistance Gene mcr-1 by ISApl1.

Author information

1
Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
2
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA.
3
Laboratoire de Microbiologie et Genetique Moleculaires, Centre National de la Recherche Scientifique, Toulouse, France.
4
Department of Laboratory Medicine, Clinical Center, Microbiology Service, National Institutes of Health, Bethesda, Maryland, USA.
5
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA dyda@helix.nih.gov.

Abstract

Analysis of mcr-1-containing sequences identified a common ∼2,607-bp DNA segment that in many cases is flanked on one or both ends by ISApl1 We present evidence that mcr-1 is mobilized by an ISApl1 composite transposon which has, in some cases, subsequently lost one or both copies of ISApl1 We also show that mcr-1 can be mobilized in some circumstances by a single upstream copy of ISApl1 in conjunction with the remnants of a downstream ISApl1.

PMID:
27620479
PMCID:
PMC5075121
DOI:
10.1128/AAC.01457-16
[Indexed for MEDLINE]
Free PMC Article

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