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Stem Cell Reports. 2016 Sep 13;7(3):383-398. doi: 10.1016/j.stemcr.2016.07.022. Epub 2016 Sep 1.

A Panel of Embryonic Stem Cell Lines Reveals the Variety and Dynamic of Pluripotent States in Rabbits.

Author information

1
Univ Lyon, Université Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, 69500 Bron, France; INRA, USC1361, 69500 Bron, France; Embryology Unit, Children's Medical Research Institute, CMRI, Westmead, NSW 2145, Australia.
2
Univ Lyon, Université Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, 69500 Bron, France.
3
ISARA-Lyon, 69007 Lyon, France; VetAgroSup, UPSP ICE, 69280 Marcy l'Etoile, France.
4
UMR BDR, INRA, ENVA, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
5
GABI, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
6
INRA, UMR 444, Génétique Cellulaire, 31076 Toulouse, France; ENVT, 31076 Toulouse, France.
7
INRA, UMR1388 Génétique, Physiologie et Systèmes d'Elevage, 31326 Castanet Tolosan, France.
8
NARIC, Agricultural Biotechnology Institute, 2100 Gödöllo, Hungary.
9
Univ Lyon, Université Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, 69500 Bron, France; INRA, USC1361, 69500 Bron, France. Electronic address: marielle.afanassieff@inserm.fr.
10
Univ Lyon, Université Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, 69500 Bron, France. Electronic address: pierre.savatier@inserm.fr.

Abstract

Conventional rabbit embryonic stem cell (ESC) lines are derived from the inner cell mass (ICM) of pre-implantation embryos using methods and culture conditions that are established for primate ESCs. In this study, we explored the capacity of the rabbit ICM to give rise to ESC lines using conditions similar to those utilized to generate naive ESCs in mice. On single-cell dissociation and culture in fibroblast growth factor 2 (FGF2)-free, serum-supplemented medium, rabbit ICMs gave rise to ESC lines lacking the DNA-damage checkpoint in the G1 phase like mouse ESCs, and with a pluripotency gene expression profile closer to the rabbit ICM/epiblast profiles. These cell lines can be converted to FGF2-dependent ESCs after culture in conventional conditions. They can also colonize the rabbit pre-implantation embryo. These results indicate that rabbit epiblast cells can be coaxed toward different types of pluripotent stem cells and reveal the dynamics of pluripotent states in rabbit ESCs.

PMID:
27594588
PMCID:
PMC5032405
DOI:
10.1016/j.stemcr.2016.07.022
[Indexed for MEDLINE]
Free PMC Article

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