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Molecules. 2016 Aug 30;21(9). pii: E1154. doi: 10.3390/molecules21091154.

Development and Validation of a Kit to Measure Drink Antioxidant Capacity Using a Novel Colorimeter.

Author information

1
Department of Biochemistry and Biotechnology, University of Thessaly, Larissa 41221, Greece. alexandros.priftis@hotmail.com.
2
Department of Biochemistry and Biotechnology, University of Thessaly, Larissa 41221, Greece. stagkos@uth.gr.
3
Polytech S.A., Larissa 41222, Greece. nikos.tzioumakis@outlook.com.
4
Coffee Island S.A., Patras 26334, Greece. kostas@coffeeisland.gr.
5
Department of Agricultural Engineering Technologists, TEI of Thessaly, Larissa 41110, Greece. anastasia.pat93@hotmail.com.
6
Department of Biochemistry and Biotechnology, University of Thessaly, Larissa 41221, Greece. geopap@bio.uth.gr.
7
Department of Forensic Sciences and Toxicology, Medical School, University of Crete, Heraklion 71003, Greece. aris@med.uoc.gr.
8
Department of Biochemistry and Biotechnology, University of Thessaly, Larissa 41221, Greece. dkouret@uth.gr.

Abstract

Measuring the antioxidant capacity of foods is essential, as a means of quality control to ensure that the final product reaching the consumer will be of high standards. Despite the already existing assays with which the antioxidant activity is estimated, new, faster and low cost methods are always sought. Therefore, we have developed a novel colorimeter and combined it with a slightly modified DPPH assay, thus creating a kit that can assess the antioxidant capacity of liquids (e.g., different types of coffee, beer, wine, juices) in a quite fast and low cost manner. The accuracy of the colorimeter was ensured by comparing it to a fully validated Hitachi U-1900 spectrophotometer, and a coefficient was calculated to eliminate the observed differences. In addition, a new, user friendly software was developed, in order to render the procedure as easy as possible, while allowing a central monitoring of the obtained results. Overall, a novel kit was developed, with which the antioxidant activity of liquids can be measured, firstly to ensure their quality and secondly to assess the amount of antioxidants consumed with the respective food.

KEYWORDS:

DPPH; antioxidant; beverage; colorimeter; drink; kit; quality control

PMID:
27589706
PMCID:
PMC6273567
DOI:
10.3390/molecules21091154
[Indexed for MEDLINE]
Free PMC Article

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