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Methods Enzymol. 2016;579:87-102. doi: 10.1016/bs.mie.2016.04.008. Epub 2016 Jun 22.

Strategies for Automated CryoEM Data Collection Using Direct Detectors.

Author information

1
Simons Electron Microscopy Center, New York Structural Biology Center, The National Resource for Automated Molecular Microscopy, New York, NY, United States.
2
Simons Electron Microscopy Center, New York Structural Biology Center, The National Resource for Automated Molecular Microscopy, New York, NY, United States; Columbia University, New York, NY, United States.
3
Simons Electron Microscopy Center, New York Structural Biology Center, The National Resource for Automated Molecular Microscopy, New York, NY, United States; Columbia University, New York, NY, United States. Electronic address: bcarr@nysbc.org.

Abstract

The new generation of direct electron detectors has been a major contributor to the recent resolution revolution in cryo-electron microscopy. Optimal use of these new cameras using automated data collection software is critical for high-throughput near-atomic resolution cryo-electron microscopy research. We present an overview of the practical aspects of automated data collection in the context of this new generation of direct detectors, highlighting the differences, challenges, and opportunities the new detectors provide compared to the previous generation of data acquisition media.

KEYWORDS:

Automated data acquisition; CryoEM; Data collection strategies; Direct detectors; Single particle methods; Throughput

PMID:
27572724
DOI:
10.1016/bs.mie.2016.04.008
[Indexed for MEDLINE]

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