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Methods Mol Biol. 2016;1432:187-202. doi: 10.1007/978-1-4939-3637-3_12.

High-Throughput Baculovirus Expression System for Membrane Protein Production.

Author information

1
New York Structural Biology Center, New York Consortium on Membrane Protein Structure (NYCOMPS), 89 Convent Avenue, New York, NY, 10027, USA. ravik@nysbc.org.
2
New York Structural Biology Center, New York Consortium on Membrane Protein Structure (NYCOMPS), 89 Convent Avenue, New York, NY, 10027, USA.

Abstract

The ease of use, robustness, cost-effectiveness, and posttranslational machinery make baculovirus expression system a popular choice for production of eukaryotic membrane proteins. This system can be readily adapted for high-throughput operations. This chapter outlines the techniques and procedures for cloning, transfection, small-scale production, and purification of membrane protein samples in a high-throughput manner.

KEYWORDS:

Bacmid; Baculovirus; Expression; High-throughput; Insect cell; Membrane protein; Nickel affinity chromatography; Purification; Sf9

PMID:
27485337
DOI:
10.1007/978-1-4939-3637-3_12
[Indexed for MEDLINE]

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