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Clin Res Hepatol Gastroenterol. 2016 Dec;40(6):748-754. doi: 10.1016/j.clinre.2016.05.006. Epub 2016 Jun 20.

MicroRNA-214 promotes peritoneal metastasis through regulating PTEN negatively in gastric cancer.

Author information

1
Ningxia Hui Autonomous Region People's Hospital, Department of Gastroenterology, Yinchuan, China.
2
Ningxia Medical University, Yinchuan, China.
3
Affiliated Hospital of Ningxia Medical University, Department of Gastroenterology, Yinchuan, China.
4
Xijing Hospital of Digestive Diseases, State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Beilin District, Changlexi Road, 710000 Xi'an, China. Electronic address: yongzhannie@yeah.net.
5
Xijing Hospital of Digestive Diseases, State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Beilin District, Changlexi Road, 710000 Xi'an, China.

Abstract

BACKGROUND AND OBJECTIVE:

We aimed to investigate the effects of microRNA-214 (miR-214) on peritoneal metastasis as well as to elucidate its regulatory mechanism in gastric cancer (GC).

METHODS:

The expression levels of miR-214 in human GC cell lines MKN-28NM, MKN-28M, GC9811 and GC9811-P were analyzed by quantitative real-time PCR. Lentiviral miR-214, lentiviral miR-214 inhibitor, and empty lentiviral vector were transfected to GC cell lines, respectively. The roles of miR-214 in cell invasion, migration, proliferation and colony-forming ability were then analyzed. Besides, the expression levels of PTEN in different transfected cells were determined by western blot analysis.

RESULTS:

We found that miR-214 was up-regulated in GC9811-P cells with high metastatic potential to the peritoneum compared with that in GC9811 cells. In addition, in vitro overexpression of miR-214 promoted cell invasion, migration, proliferation and colony-forming ability of GC9811 cells, while down-regulation of miR-214 had opposite effects in GC9811-P cells. Besides, overexpression of miR-214 in GC9811 cells markedly down-regulated PTEN expression, whereas down-regulation of miR-214 in GC9811-P cells significantly increased PTEN expression.

CONCLUSIONS:

Our findings indicate that miR-214 may promote peritoneal metastasis of GC cells via down-regulation of PTEN, thus leading to the progression of GC.

PMID:
27339596
DOI:
10.1016/j.clinre.2016.05.006
[Indexed for MEDLINE]

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