Background: Knowledge of the composition of vaginal microbial ecosystem is essential for understanding the etiology, prevention, and treatment of vaginal diseases. A baboon model has been used to provide detailed understanding of reproductive physiology and immunology applicable to women. However, little is known about the composition of its vaginal microbial ecosystem.
Methods: Gram stain and Nugent scores were used for assessment of baboon vaginal microbial flora. Biochemical identification and analysis of isolates were performed using the api(®) kits and identification software.
Results: Species of Lactobacilli, Staphylococci, Clostridia, Bacilli, Corynebacteria, Gram-negative rods, other Gram-positive rods, cocci and Candida, were isolated. Healthy vaginal microbiota consisted mainly of lactobacillus morphotypes. Animals with high Nugent scores had increased number of Gram-positive cocci and variable rods, with increased number of Gram-negative morphotypes.
Conclusions: The baboon vaginal microbiota is heterogeneous in terms of species composition and is typified by a scarcity of lactobacilli.
Keywords: Papio anubis; bacteria; cultulre; non-human primates; reproductive tract.
© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.