Format

Send to

Choose Destination
Genes Dev. 2016 May 15;30(10):1155-71. doi: 10.1101/gad.280941.116. Epub 2016 May 19.

Evolution of a transcriptional regulator from a transmembrane nucleoporin.

Author information

1
Laboratory of Molecular and Cellular Biology, Salk Institute for Biological Studies, La Jolla, California 92037, USA;
2
Laboratory of Genetics, Salk Institute for Biological Studies, La Jolla, California 92037, USA;
3
Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA;
4
Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA; Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA;
5
Laboratory of Genetics, Salk Institute for Biological Studies, La Jolla, California 92037, USA; Center for Academic Research and Training in Anthropogeny (CARTA), La Jolla, California 92093, USA.

Abstract

Nuclear pore complexes (NPCs) emerged as nuclear transport channels in eukaryotic cells ∼1.5 billion years ago. While the primary role of NPCs is to regulate nucleo-cytoplasmic transport, recent research suggests that certain NPC proteins have additionally acquired the role of affecting gene expression at the nuclear periphery and in the nucleoplasm in metazoans. Here we identify a widely expressed variant of the transmembrane nucleoporin (Nup) Pom121 (named sPom121, for "soluble Pom121") that arose by genomic rearrangement before the divergence of hominoids. sPom121 lacks the nuclear membrane-anchoring domain and thus does not localize to the NPC. Instead, sPom121 colocalizes and interacts with nucleoplasmic Nup98, a previously identified transcriptional regulator, at gene promoters to control transcription of its target genes in human cells. Interestingly, sPom121 transcripts appear independently in several mammalian species, suggesting convergent innovation of Nup-mediated transcription regulation during mammalian evolution. Our findings implicate alternate transcription initiation as a mechanism to increase the functional diversity of NPC components.

KEYWORDS:

Nup98; Pom121; evolution; hominoid; nuclear pore complex (NPC); transcription

PMID:
27198230
PMCID:
PMC4888837
DOI:
10.1101/gad.280941.116
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center