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Cell Rep. 2016 Mar 29;14(12):2833-45. doi: 10.1016/j.celrep.2016.02.081. Epub 2016 Mar 17.

Structural Model of the Extracellular Assembly of the TCR-CD3 Complex.

Author information

1
Laura and Isaac Perlmutter Cancer Center, New York University School of Medicine, New York, NY 10016, USA.
2
Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016, USA.
3
Department of Biochemistry, Weill Cornell Medical College, New York, NY 10065, USA.
4
Laura and Isaac Perlmutter Cancer Center, New York University School of Medicine, New York, NY 10016, USA; Interdiciplinary Cooperative Melanoma Group, New York University School of Medicine, New York, NY 10016, USA; Department of Pathology, New York University School of Medicine, New York, NY 10016, USA. Electronic address: michelle.krogsgaard@nyumc.org.

Abstract

Antigen recognition of peptide-major histocompatibility complexes (pMHCs) by T cells, a key step in initiating adaptive immune responses, is performed by the T cell receptor (TCR) bound to CD3 heterodimers. However, the biophysical basis of the transmission of TCR-CD3 extracellular interaction into a productive intracellular signaling sequence remains incomplete. Here we used nuclear magnetic resonance (NMR) spectroscopy combined with mutational analysis and computational docking to derive a structural model of the extracellular TCR-CD3 assembly. In the inactivated state, CD3γε interacts with the helix 3 and helix 4-F strand regions of the TCR Cβ subunit, whereas CD3δε interacts with the F and C strand regions of the TCR Cα subunit in this model, placing the CD3 subunits on opposing sides of the TCR. This work identifies the molecular contacts between the TCR and CD3 subunits, identifying a physical basis for transmitting an activating signal through the complex.

PMID:
26997265
PMCID:
PMC4902171
DOI:
10.1016/j.celrep.2016.02.081
[Indexed for MEDLINE]
Free PMC Article

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