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Nat Protoc. 2016 Apr;11(4):747-63. doi: 10.1038/nprot.2016.040. Epub 2016 Mar 17.

A LC-MS-based workflow for measurement of branched fatty acid esters of hydroxy fatty acids.

Author information

1
Salk Institute for Biological Studies, La Jolla, California, USA.
2
Division of Endocrinology, Diabetes &Metabolism, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA.
3
Wallenberg Laboratory, Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg, Sweden.
4
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.
5
Lundberg Laboratory for Diabetes Research, Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg, Sweden.

Abstract

Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of endogenous mammalian lipids with antidiabetic and anti-inflammatory effects. We previously identified 16 different FAHFA families, such as branched palmitic acid esters of hydroxy stearic acids (PAHSAs); each family consists of multiple isomers in which the branched ester is at different positions (e.g., 5- and 9-PAHSA). We anticipate increased need for PAHSA measurements as markers of metabolic and inflammatory health. In this protocol, we provide a detailed description of the extraction of FAHFAs from human or mouse tissues, their enrichment by solid-phase extraction and subsequent analysis by LC-MS. For a sample size of 6-12, the time frame is 2-3 d.

PMID:
26985573
PMCID:
PMC4797065
DOI:
10.1038/nprot.2016.040
[Indexed for MEDLINE]
Free PMC Article

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