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Biomarkers. 2016 Jul;21(5):458-65. doi: 10.3109/1354750X.2016.1153721. Epub 2016 Mar 17.

Peripheral blood collection: the first step towards gene expression profiling.

Author information

1
a Unit Environmental Risk and Health, Flemish Institute for Technological Research (VITO NV) , Mol , Belgium ;
2
b Department of Biomedical Sciences ;
3
c Department of Epidemiology and Social Medicine , University of Antwerp , Antwerp , Belgium ;
4
d Department of Environmental Medicine , University of Southern Denmark , Odense , Denmark.

Abstract

A crucial challenge for gene expression analysis in human biomonitoring studies on whole blood samples is rapid sample handling and mRNA stabilization. This study was designed to evaluate the impact of short bench times (less than 30 min) on yield, quality and gene expression of mRNA in the presence of different stabilization buffers (Tempus(TM) Blood RNA tube and RNAlater(®) Stabilization Reagent). Microarray analyzes showed significant changes over short periods of time in expression of a considerate part of the transcriptome (2356 genes) with a prominent role for NFкB-, cancer- and glucocorticoid-mediated networks, and specifically interleukin-8 (IL-8). These findings suggest that even short bench times affect gene expression, requiring to carry out blood collection in a strictly standardized way.

KEYWORDS:

Bench time; IL-8; RNA stability; biomarker; human blood sampling

PMID:
26984061
DOI:
10.3109/1354750X.2016.1153721
[Indexed for MEDLINE]

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