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J Lab Autom. 2016 Oct;21(5):706-12. doi: 10.1177/2211068216634003. Epub 2016 Feb 26.

Ultra-High-Throughput Sample Preparation System for Lymphocyte Immunophenotyping Point-of-Care Diagnostics.

Author information

1
Science for Life Laboratory, Division of Proteomics and Nanobiotechnology, KTH Royal Institute of Technology, Stockholm, Sweden Department of Bioengineering, Northeastern University, Boston, MA, USA.
2
Department of Chemical Engineering, Northeastern University, Boston, MA, USA Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, MA, USA.
3
Science for Life Laboratory, Division of Proteomics and Nanobiotechnology, KTH Royal Institute of Technology, Stockholm, Sweden aman.russom@scilifelab.se.

Abstract

Point-of-care (POC) microfluidic devices often lack the integration of common sample preparation steps, such as preconcentration, which can limit their utility in the field. In this technology brief, we describe a system that combines the necessary sample preparation methods to perform sample-to-result analysis of large-volume (20 mL) biopsy model samples with staining of captured cells. Our platform combines centrifugal-paper microfluidic filtration and an analysis system to process large, dilute biological samples. Utilizing commercialization-friendly manufacturing methods and materials, yielding a sample throughput of 20 mL/min, and allowing for on-chip staining and imaging bring together a practical, yet powerful approach to microfluidic diagnostics of large, dilute samples.

KEYWORDS:

cell capture; cell immunophenotyping; centrifugal microfluidics; paper-based microfluidics; sample preparation

PMID:
26920576
DOI:
10.1177/2211068216634003
[Indexed for MEDLINE]

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