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Gen Pharmacol. 1989;20(5):609-14.

Cloning of mercury-resistance gene from R-plasmid in Escherichia coli isolated from dental hospital sewage.

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Department of Biochemistry, Nihon University School of Dentistry, Chiba, Japan.


1. Mercury-resistance gene was cloned from a drug-resistance plasmid in Escherichia coli isolated from dental hospital sewage. 2. A 7.7 kb mercury-resistance gene was successfully cloned and expressed in pJC74 cosmid and pBR 322 plasmid vectors. 3. High activities of mercury reductase and 203Hg vaporization were demonstrated in cell extracts of mercury-resistant clone. 4. Mercury reductase activity was localized in the cytoplasm. 5. By colony DNA-DNA hybridization, 32P-labelled 7.7-kb DNA hybridized with mercury-resistant E. coli isolates from dental hospital sewage.

[Indexed for MEDLINE]

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