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Methods Mol Biol. 2016;1395:281-98. doi: 10.1007/978-1-4939-3347-1_16.

Proteomics in the Assessment of the Therapeutic Response of Antineoplastic Drugs: Strategies and Practical Applications.

Author information

1
Laboratory of Proteomics, Human Genetics Departament, Instituto Nacional de Saúde Dr Ricardo Jorge, Av. Padre Cruz, Lisbon, 1649-016, Portugal.
2
ToxOmics-Centre of Toxicogenomics and Human Health, Universidade Nova de Lisboa, Lisboa, Portugal.
3
Medical Oncology Department, Oncology Institute of Vojvodina, Sremska Kamenica, Serbia.
4
Medical Faculty, University of Novi Sad, Novi Sad, Serbia.
5
Laboratory of Proteomics, Human Genetics Departament, Instituto Nacional de Saúde Dr Ricardo Jorge, Av. Padre Cruz, Lisbon, 1649-016, Portugal. deborah.penque@insa.min-saude.pt.
6
ToxOmics-Centre of Toxicogenomics and Human Health, Universidade Nova de Lisboa, Lisboa, Portugal. deborah.penque@insa.min-saude.pt.

Abstract

Uncovering unknown pathological mechanisms and body response to applied medication are the driving forces toward personalized medicine. In this post-genomic era, all eyes are turned to the proteomics field, searching for answers and explanations by investigating the gene end point functional units-proteins and their proteoforms. The development of cutting-edge mass spectrometric technologies and bioinformatics tools have allowed the life-science community to discover disease-specific proteins as biomarkers, which are often concealed by high sample complexity and dynamic range of abundance. Currently, there are several proteomics-based approaches to investigate the proteome. This chapter focuses on gold standard proteomics strategies and related issues toward candidate biomarker discovery, which may have diagnostic/prognostic as well as mechanistic utility in cancer drug resistance.

KEYWORDS:

Cancer; Chemotherapeutic response; Discovery-based proteomics; Proteomics; Target-proteomics

PMID:
26910080
DOI:
10.1007/978-1-4939-3347-1_16
[Indexed for MEDLINE]

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