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Sci Rep. 2016 Jan 29;6:19993. doi: 10.1038/srep19993.

Translation Microscopy (TRAM) for super-resolution imaging.

Author information

1
Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh, EH14 4AS.
2
Edinburgh Super-Resolution Imaging Consortium, Heriot-Watt University, Edinburgh, EH14 4AS.
3
Department of Chemistry, Tsinghua University, Beijing, China.
4
University of Edinburgh, Western General Hospital, Crewe Road South, Edinburgh, EH4 2XR.

Abstract

Super-resolution microscopy is transforming our understanding of biology but accessibility is limited by its technical complexity, high costs and the requirement for bespoke sample preparation. We present a novel, simple and multi-color super-resolution microscopy technique, called translation microscopy (TRAM), in which a super-resolution image is restored from multiple diffraction-limited resolution observations using a conventional microscope whilst translating the sample in the image plane. TRAM can be implemented using any microscope, delivering up to 7-fold resolution improvement. We compare TRAM with other super-resolution imaging modalities, including gated stimulated emission deletion (gSTED) microscopy and atomic force microscopy (AFM). We further developed novel 'ground-truth' DNA origami nano-structures to characterize TRAM, as well as applying it to a multi-color dye-stained cellular sample to demonstrate its fidelity, ease of use and utility for cell biology.

PMID:
26822455
PMCID:
PMC4731806
DOI:
10.1038/srep19993
[Indexed for MEDLINE]
Free PMC Article

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