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Am J Reprod Immunol. 2016 Apr;75(4):486-92. doi: 10.1111/aji.12489. Epub 2016 Jan 19.

Resveratrol Enhances Apoptosis in Endometriotic Stromal Cells.

Author information

1
Department of Obstetrics and Gynecology, Faculty of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.

Abstract

PROBLEM:

Resistance to apoptosis, together with inflammatory and invasive activity, contributes to the pathogenesis of endometriosis; therefore, approaches that can safely enhance apoptosis in endometriotic tissue are highly sought after as a means of managing the disease. Although resveratrol (RVT) is known to induce apoptosis or increase sensitivity to apoptotic stimuli in various cancer cell types, its effect on human endometriosis has remained uncertain. This study aimed to investigate whether RVT induces or enhances apoptosis in human endometriotic stromal cells (ESCs).

METHOD OF STUDY:

Endometriotic tissues were collected, during laparoscopies, from women affected by ovarian endometriosis. ESCs were prepared, cultured, and treated with RVT. Apoptosis was assessed by annexin V-PI staining. Survivin mRNA expression in ESCs was examined using RT-PCR. ESCs were pre-treated with or without RVT and then incubated with TNF-α-related-apoptosis-inducing ligand (TRAIL), which is a known pro-apoptotic molecule.

RESULTS:

RVT alone did not induce apoptosis in ESCs. RVT significantly reduced survivin mRNA expression (P < 0.05). Pre-treatment with RVT significantly enhanced TRAIL-induced apoptosis (8.13 ± 0.83% (control) versus 29.19 ± 7.39% (pre-treated with RVT), P < 0.05).

CONCLUSION:

This study indicates that RVT suppresses survivin expression and enhances TRAIL-induced apoptosis in ESCs.

KEYWORDS:

Anti-apoptosis; TNF-α-related-apoptosis-inducing ligand; Therapy; endometriosis; survivin

PMID:
26782781
DOI:
10.1111/aji.12489
[Indexed for MEDLINE]

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