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Methods Mol Biol. 2016;1354:49-59. doi: 10.1007/978-1-4939-3046-3_4.

Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core Integrity.

Author information

1
Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 303 E. Superior, Lurie 9-280, Chicago, IL, 60611, USA.
2
Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 303 E. Superior, Lurie 9-280, Chicago, IL, 60611, USA. thope@northwestern.edu.

Abstract

Live cell imaging is a valuable technique that allows the characterization of the dynamic processes of the HIV-1 life cycle. Here, we present a method of production and imaging of dual-labeled HIV viral particles that allows the visualization of two events. Varying release of the intravirion fluid phase marker reveals virion fusion and the loss of the integrity of HIV viral cores with the use of live wide-field fluorescent microscopy.

KEYWORDS:

HIV early steps of infection; HIV fusion; HIV uncoating; HIV viral core; Live cell imaging; Wide-field microscopy

PMID:
26714704
PMCID:
PMC4876021
DOI:
10.1007/978-1-4939-3046-3_4
[Indexed for MEDLINE]
Free PMC Article

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