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Biomed Opt Express. 2015 Nov 19;6(12):4923-33. doi: 10.1364/BOE.6.004923. eCollection 2015 Dec 1.

Quantitative spatial frequency fluorescence imaging in the sub-diffusive domain for image-guided glioma resection.

Author information

1
Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, ON M5G 1L7 Canada ; Princess Margaret Cancer Center/University Health Network, 610 University Avenue, Toronto, ON M5G 2M3 Canada.
2
Princess Margaret Cancer Center/University Health Network, 610 University Avenue, Toronto, ON M5G 2M3 Canada.
3
Thayer School of Engineering at Dartmouth, Thayer School of Engineering at Dartmouth 14 Engineering Drive Hanover, NH USA 03755 USA.
4
École Polytechnique De Montreal, 2900, boul. Édouard-Montpetit Montréal, Québec H3T 1J4 Canada.

Abstract

Intraoperative 5- aminolevulinic acid induced-Protoporphyrin IX (PpIX) fluorescence guidance enables maximum safe resection of glioblastomas by providing surgeons with real-time tumor optical contrast. However, visual assessment of PpIX fluorescence is subjective and limited by the distorting effects of light attenuation and tissue autofluorescence. We have previously shown that non-invasive point measurements of absolute PpIX concentration identifies residual tumor that is otherwise non-detectable. Here, we extend this approach to wide-field quantitative fluorescence imaging by implementing spatial frequency domain imaging to recover tissue optical properties across the field-of-view in phantoms and ex vivo tissue.

KEYWORDS:

(110.0110) Imaging systems; (110.0113) Imaging through turbid media; (170.6510) Spectroscopy, tissue diagnostics

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