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Sci Rep. 2015 Nov 3;5:15915. doi: 10.1038/srep15915.

SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy.

Author information

1
Department of Biochemistry, University of Oxford, Oxford, UK.
2
Division of Structural Biology, Wellcome Trust Centre for Human Genetics University of Oxford, Oxford, UK.

Abstract

Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data, and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms, and will promote routine application of super-resolution SIM imaging in cell biology.

PMID:
26525406
PMCID:
PMC4648340
DOI:
10.1038/srep15915
[Indexed for MEDLINE]
Free PMC Article

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