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Biotechnol Biofuels. 2015 Oct 20;8:169. doi: 10.1186/s13068-015-0353-5. eCollection 2015.

Inducible gene expression system by 3-hydroxypropionic acid.

Author information

1
School of Chemical and Biomolecular Engineering, Pusan National University, San 30 Jangeon-dong, Geumjeong-gu, Busan, 609-735 Republic of Korea.
2
Department of Biochemical Engineering, College of Pharmaceutical and Life Sciences, Changzhou University, Changzhou, 213164 China.
#
Contributed equally

Abstract

BACKGROUND:

3-Hydroxypropionic acid (3-HP) is an important platform chemical that boasts a variety of industrial applications. Gene expression systems inducible by 3-HP, if available, are of great utility for optimization of the pathways of 3-HP production and excretion.

RESULTS:

Here we report the presence of unique inducible gene expression systems in Pseudomonas denitrificans and other microorganisms. In P. denitrificans, transcription of three genes (hpdH, mmsA and hbdH-4) involved in 3-HP degradation was upregulated by 3-HP by the action of a transcriptional regulator protein, LysR, and a cis-acting regulatory site for LysR binding. Similar inducible systems having an LysR transcriptional regulator were identified in other microorganisms that also could degrade 3-HP. A docking study showed that the 3-HP binding pocket is located between the N-terminal helix-turn-helix motif and the C-terminal cofactor-binding domain.

CONCLUSIONS:

This LysR-regulated 3-HP-inducible system should prove useful for control of the level of gene expression in response to 3-HP.

KEYWORDS:

3-Hydroxypropionic acid; Inducible; LysR; hbdH; hpdH; mmsA

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