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Cold Spring Harb Protoc. 2015 Oct 1;2015(10):925-31. doi: 10.1101/pdb.prot086371.

Purification and Injection of Retroviral Vectors.

Abstract

Retroviral vectors are powerful tools for genetic manipulation. This protocol discusses the production, purification, and use of replication-deficient retroviral vectors based on Moloney murine leukemia virus and lentivirus. It also describes the injection of a retroviral vector into the dentate gyrus of young adult mice to fluorescently label live murine brain tissue.

PMID:
26430255
DOI:
10.1101/pdb.prot086371
[Indexed for MEDLINE]

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