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Exp Eye Res. 2016 Feb;143:9-16. doi: 10.1016/j.exer.2015.09.014. Epub 2015 Sep 28.

Topical trans-resveratrol ameliorates steroid-induced anterior and posterior segment changes in rats.

Author information

1
Faculty of Medicine, Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia. Electronic address: norhafiza8409@salam.uitm.edu.my.
2
Faculty of Medicine, Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia.
3
School of Medicine, International Medical University, IMU Clinical School, Department of Ophthalmology, Jalan Rasah, Seremban, Malaysia.
4
Universiti Teknologi MARA, Faculty of Pharmacy, Dept. of Pharmaceutics, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia.
5
Pathological Anatomy Department, Volgograd State Medical University, 1, Pavshikh Bortsov Square, Volgograd, 400131, Russia.

Abstract

Steroid-induced hypertension and glaucoma is associated with increased extracellular meshwork (ECM) deposition in trabecular meshwork (TM). Previous studies have shown that single drop application of trans-resveratrol lowers IOP in steroid-induced ocular hypertensive (SIOH) rats. This IOP lowering is attributed to activation of adenosine A1 receptors, which may lead to increased matrix metalloproteinase (MMP)-2 activity. This study evaluated the effect of repeated topical application of trans-resveratrol for 21 days in SIOH animals on IOP, changes in MMP-2 level in aqueous humor, trabecular meshwork and retinal morphology and retinal redox status. We observed that treatment with trans-resveratrol results in significant and sustained IOP reduction in SIOH rats. This IOP reduction is associated with significantly higher aqueous humor total MMP-2 level; significantly reduced TM thickness and increased number of TM cells. Treatment with trans-resveratrol also significantly increased ganglion cell layer (GCL) thickness, the linear cell density in the GCL and inner retina thickness; and significantly reduced retinal oxidative stress compared to the SIOH vehicle-treated group. In conclusion, repeated dose topical application of trans-resveratrol produces sustained IOP lowering effect, which is associated with increased level of aqueous humor MMP-2, normalization of TM and retinal morphology and restoration of retinal redox status.

KEYWORDS:

Glaucoma; Intraocular pressure; Resveratrol; Retina; Steroid-induced; Trabecular meshwork

PMID:
26424219
DOI:
10.1016/j.exer.2015.09.014
[Indexed for MEDLINE]

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