Abstract
Protein tagging is widely used in approaches ranging from affinity purification to fluorescence-based detection in live cells. However, an intrinsic limitation of tagging is that the native function of the protein may be compromised or even abolished by the presence of the tag. Here we describe and characterize a set of small, innocuous protein tags (inntags) that we anticipate will find application in a variety of biological techniques.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal
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Epitopes / analysis*
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Epitopes / chemistry*
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Epitopes / genetics
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Female
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Fluorescent Antibody Technique / methods*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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HEK293 Cells
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Humans
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Immunoprecipitation / methods*
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Mice
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Mice, Inbred BALB C
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NIH 3T3 Cells
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Protein Conformation
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Protein Structure, Tertiary
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Proteins / analysis*
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Proteins / immunology*
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
Substances
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Antibodies, Monoclonal
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Epitopes
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Proteins
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Recombinant Proteins
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Green Fluorescent Proteins