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Alcohol. 2015 Nov;49(7):707-12. doi: 10.1016/j.alcohol.2015.04.010. Epub 2015 Jul 21.

Anxiety-like behaviors at the end of the nocturnal period in sP rats with a "history" of unpredictable, limited access to alcohol.

Author information

1
Neuroscience Institute, Section of Cagliari, National Research Council of Italy, S.S. 554, km. 4,500, I-09042 Monserrato, CA, Italy. Electronic address: colomb@unica.it.
2
Neuroscience Institute, Section of Cagliari, National Research Council of Italy, S.S. 554, km. 4,500, I-09042 Monserrato, CA, Italy.
3
Neuroscience Institute, Section of Cagliari, National Research Council of Italy, S.S. 554, km. 4,500, I-09042 Monserrato, CA, Italy; Department of Biomedical Sciences, University of Cagliari, S.S. 554, km. 4,500, I-09042 Monserrato, CA, Italy.
4
Department of Biomedical Sciences, University of Sassari, Via Muroni 23, I-07100 Sassari, SS, Italy.
5
Department of Biotechnologies, Chemistry, and Pharmacy, University of Siena, Via Aldo Moro 2, I-53100 Siena, SI, Italy.

Abstract

Recent research found that exposure of selectively bred, Sardinian alcohol-preferring (sP) rats to multiple alcohol concentrations (10%, 20%, and 30%, v/v), under the 4-bottle "alcohol vs. water" choice regimen, in daily 1-h drinking sessions with an unpredictable time schedule, promoted high intakes of alcohol (≥2 g/kg) when the drinking session occurred over the final hours of the dark phase of the light/dark cycle. The present study investigated whether these high intakes of alcohol (a) were associated with alterations in rats' emotional state (Experiment 1) and (b) were pharmacologically manipulable (Experiment 2). In both experiments, over a period of 12 days, sP rats were initially exposed daily to a 1-h drinking session during the dark phase; time of alcohol exposure was changed each day and was unpredictable to rats. The day after this 12-day drinking phase, rats were (a) exposed to the Social Interaction (SI) test at the 1st or 12th hour of the dark phase with no alcohol available (Experiment 1) or (b) treated with the positive allosteric modulator of the GABAB receptor, GS39783 (0, 25, 50, and 100 mg/kg, intragastrically [i.g.]), and exposed to a drinking session at the 12th hour of the dark phase (Experiment 2). In Experiment 1, rats exposed to the SI test during the 12th hour spent approximately 35% less time in "social" behaviors than rats exposed to the SI test during the 1st hour. No difference in "social" behaviors was observed between alcohol-naive sP rats exposed to the SI test at the 1st and 12th hour. In Experiment 2, all doses of GS39783 selectively reduced alcohol intake. These results suggest that (a) expectation of alcohol availability likely exacerbated the anxiety-like state of sP rats and (b) the GABAB receptor is part of the neural substrate underlying these exceptionally high intakes of alcohol in sP rats.

KEYWORDS:

Anxiety-like behaviors; Experimental model of binge drinking; Limited and unpredictable access to alcohol; Positive allosteric modulator of the GABA(B) receptor, GS39783; Sardinian alcohol-preferring (sP) rats; Time schedule of alcohol drinking

PMID:
26254964
PMCID:
PMC4636466
DOI:
10.1016/j.alcohol.2015.04.010
[Indexed for MEDLINE]
Free PMC Article

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