Genome-Wide Copy Number Variations Using SNP Genotyping in a Mixed Breed Swine Population

PLoS One. 2015 Jul 14;10(7):e0133529. doi: 10.1371/journal.pone.0133529. eCollection 2015.

Abstract

Copy number variations (CNVs) are increasingly understood to affect phenotypic variation. This study uses SNP genotyping of trios of mixed breed swine to add to the catalog of known genotypic variation in an important agricultural animal. PorcineSNP60 BeadChip genotypes were collected from 1802 pigs that combined to form 1621 trios. These trios were from the crosses of 50 boars with 525 sows producing 1621 piglets. The pigs were part of a population that was a mix of ¼ Duroc, ½ Landrace and ¼ Yorkshire breeds. Merging the overlapping CNVs that were observed in two or more individuals to form CNV regions (CNVRs) yielded 502 CNVRs across the autosomes. The CNVRs intersected genes, as defined by RefSeq, 84% of the time - 420 out of 502. The results of this study are compared and contrasted to other swine studies using similar and different methods of detecting CNVR. While progress is being made in this field, more work needs to be done to improve consistency and confidence in CNVR results.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Breeding / methods
  • DNA Copy Number Variations / genetics*
  • Female
  • Genome / genetics
  • Genotype
  • Male
  • Polymorphism, Single Nucleotide / genetics*
  • Swine / genetics*

Grants and funding

This work was funded by Current Research Information System #5438-31000-083-00D of the Agricultural Research Service, a division of the US Department of Agriculture. The funder did not contribute to the study design, data collation and analysis, decision to publish, or preparation of the manuscript.