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Cell Rep. 2015 Jul 21;12(3):482-94. doi: 10.1016/j.celrep.2015.06.043. Epub 2015 Jul 9.

OTX2 Transcription Factor Controls Regional Patterning within the Medial Ganglionic Eminence and Regional Identity of the Septum.

Author information

1
Department of Psychiatry, University of California, San Francisco, San Francisco, CA 94143, USA.
2
Department of Psychiatry, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address: john.rubenstein@ucsf.edu.

Abstract

The Otx2 homeodomain transcription factor is essential for gastrulation and early neural development. We generated Otx2 conditional knockout (cKO) mice to investigate its roles in telencephalon development after neurulation (approximately embryonic day 9.0). We conducted transcriptional profiling and in situ hybridization to identify genes de-regulated in Otx2 cKO ventral forebrain. In parallel, we used chromatin immunoprecipitation sequencing to identify enhancer elements, the OTX2 binding motif, and de-regulated genes that are likely direct targets of OTX2 transcriptional regulation. We found that Otx2 was essential in septum specification, regulation of Fgf signaling in the rostral telencephalon, and medial ganglionic eminence (MGE) patterning, neurogenesis, and oligodendrogenesis. Within the MGE, Otx2 was required for ventral, but not dorsal, identity, thus controlling the production of specific MGE derivatives.

PMID:
26166575
PMCID:
PMC4512845
DOI:
10.1016/j.celrep.2015.06.043
[Indexed for MEDLINE]
Free PMC Article

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