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PLoS One. 2015 Jul 1;10(7):e0131643. doi: 10.1371/journal.pone.0131643. eCollection 2015.

Improved Angiogenesis in Response to Localized Delivery of Macrophage-Recruiting Molecules.

Author information

1
Graduate Program in Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, Houston, Texas, United States of America; Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, United States of America.
2
Department of Bioengineering, Rice University, Houston, Texas, United States of America.
3
Department of Bioengineering, Rice University, Houston, Texas, United States of America; Department of Biomedical Engineering, Duke University, Durham, North Carolina, United States of America.
4
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, United States of America.
5
Graduate Program in Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, Houston, Texas, United States of America; Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, United States of America; Cardiovascular Research Institute, Baylor College of Medicine, Houston, Texas, United States of America.
6
Graduate Program in Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, Houston, Texas, United States of America; Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, United States of America; Cardiovascular Research Institute, Baylor College of Medicine, Houston, Texas, United States of America; Department of Bioengineering, Rice University, Houston, Texas, United States of America.

Abstract

Successful engineering of complex organs requires improved methods to promote rapid and stable vascularization of artificial tissue scaffolds. Toward this goal, tissue engineering strategies utilize the release of pro-angiogenic growth factors, alone or in combination, from biomaterials to induce angiogenesis. In this study we have used intravital microscopy to define key, dynamic cellular changes induced by the release of pro-angiogenic factors from polyethylene glycol diacrylate hydrogels transplanted in vivo. Our data show robust macrophage recruitment when the potent and synergistic angiogenic factors, PDGFBB and FGF2 were used as compared with VEGF alone and intravital imaging suggested roles for macrophages in endothelial tip cell migration and anastomosis, as well as pericyte-like behavior. Further data from in vivo experiments show that delivery of CSF1 with VEGF can dramatically improve the poor angiogenic response seen with VEGF alone. These studies show that incorporating macrophage-recruiting factors into the design of pro-angiogenic biomaterial scaffolds is a key strategy likely to be necessary for stable vascularization and survival of implanted artificial tissues.

PMID:
26132702
PMCID:
PMC4489184
DOI:
10.1371/journal.pone.0131643
[Indexed for MEDLINE]
Free PMC Article

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