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Planta. 2015 Nov;242(5):1195-206. doi: 10.1007/s00425-015-2354-9. Epub 2015 Jul 1.

Expression of a rice glutaredoxin in aleurone layers of developing and mature seeds: subcellular localization and possible functions in antioxidant defense.

Author information

1
Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, Kyoto, 606-8522, Japan. s_morita@kpu.ac.jp.
2
Biotechnology Research Department, Kyoto Prefectural Agriculture, Forestry and Fisheries Technology Center, Seika, Soraku, Kyoto, 619-0244, Japan. s_morita@kpu.ac.jp.
3
Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, Kyoto, 606-8522, Japan.
4
Radiation Effect Accumulation and Prevention Project, Fukushima Project Headquarters, National Institute of Radiological Sciences, Anagawa, Inage, Chiba, 263-8555, Japan.
5
Laboratory of Plant Molecular Biology, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, Kyoto, 606-8522, Japan.
6
Graduate School of Biostudies, Kyoto University, Kyoto, Sakyo, 606-8501, Japan.
7
Agricultural Faculty, Tadulako University, Palu, 94118, Indonesia.
8
Biotechnology Research Department, Kyoto Prefectural Agriculture, Forestry and Fisheries Technology Center, Seika, Soraku, Kyoto, 619-0244, Japan.
9
Kihara Institute for Biological Research, Yokohama City University, Totsuka, Yokohama, 244-0813, Japan.
10
Faculty of Agriculture, Ryukoku University, Seta, Otsu, 520-2194, Japan.

Abstract

A rice glutaredoxin isoform (OsGrxC2;2) with antioxidant capacity is expressed abundantly in seed tissues and is localized to storage vacuoles in aleurone layers in developing and mature seeds. Seed tissues undergo drastic water loss at the late stage of seed development, and thus need to tolerate oxidative injuries associated with desiccation. We previously found a rice glutaredoxin isoform, OsGrxC2;2, as a gene expressed abundantly in developing seeds. Since glutaredoxin is involved in antioxidant defense, in the present study we investigated the subcellular localization and expression profile of OsGrxC2;2 and whether OsGrxC2;2 has a role in the defense against reactive oxygen species. Western blotting and immunohistochemistry revealed that the OsGrxC2;2 protein accumulated at a high level in the embryo and aleurone layers of developing and mature seeds. The OsGrxC2;2 in developing seeds was particularly localized to aleurone grains, which are storage organelles derived from vacuoles. Overexpression of OsGrxC2;2 resulted in an enhanced tolerance to menadione in yeast and methyl viologen in green leaves of transgenic rice plants. These results suggest that OsGrxC2;2 participates in the defense against oxidative stress in developing and mature seeds.

KEYWORDS:

Aleurone layers; Antioxidative defense; Glutaredoxin; Oryza; Oxidative stress

PMID:
26126957
DOI:
10.1007/s00425-015-2354-9
[Indexed for MEDLINE]

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