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Nat Methods. 2015 Aug;12(8):767-72. doi: 10.1038/nmeth.3453. Epub 2015 Jun 29.

Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome.

Author information

1
Department of Pharmacology, Weill Medical College, Cornell University, New York, New York, USA.
2
1] Department of Physiology and Biophysics, Weill Medical College, Cornell University, New York, New York, USA. [2] HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Medical College, Cornell University, New York, New York, USA.

Abstract

N(6)-methyladenosine (m6A) is the most abundant modified base in eukaryotic mRNA and has been linked to diverse effects on mRNA fate. Current mapping approaches localize m6A residues to transcript regions 100-200 nt long but cannot identify precise m6A positions on a transcriptome-wide level. Here we developed m6A individual-nucleotide-resolution cross-linking and immunoprecipitation (miCLIP) and used it to demonstrate that antibodies to m6A can induce specific mutational signatures at m6A residues after ultraviolet light-induced antibody-RNA cross-linking and reverse transcription. We found that these antibodies similarly induced mutational signatures at N(6),2'-O-dimethyladenosine (m6Am), a modification found at the first nucleotide of certain mRNAs. Using these signatures, we mapped m6A and m6Am at single-nucleotide resolution in human and mouse mRNA and identified small nucleolar RNAs (snoRNAs) as a new class of m6A-containing non-coding RNAs (ncRNAs).

PMID:
26121403
PMCID:
PMC4487409
DOI:
10.1038/nmeth.3453
[Indexed for MEDLINE]
Free PMC Article

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