Draft Genome Sequence of the Bacterium Pseudomonas putida CBB5, Which Can Utilize Caffeine as a Sole Carbon and Nitrogen Source

Erik M Quandt; Ryan M Summers; Mani V Subramanian; Jeffrey E Barrick

doi:10.1128/genomeA.00640-15

Draft Genome Sequence of the Bacterium Pseudomonas putida CBB5, Which Can Utilize Caffeine as a Sole Carbon and Nitrogen Source

Genome Announc. 2015 Jun 11;3(3):e00640-15. doi: 10.1128/genomeA.00640-15.

Authors

Erik M Quandt¹, Ryan M Summers², Mani V Subramanian³, Jeffrey E Barrick⁴

Affiliations

¹ Center for Systems and Synthetic Biology, Center for Computational Biology and Bioinformatics, Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas, USA.
² Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, Alabama, USA.
³ Center for Biocatalysis and Bioprocessing, Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, Iowa, USA.
⁴ Center for Systems and Synthetic Biology, Center for Computational Biology and Bioinformatics, Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas, USA jbarrick@cm.utexas.edu.

Abstract

Pseudomonas putida CBB5 was isolated from soil by enriching for growth on caffeine (1,3,7-trimethylxanthine). The draft genome of this strain is 6.9 Mb, with 5,941 predicted coding sequences. It includes the previously studied Alx gene cluster encoding alkylxanthine N-demethylase enzymes and other genes that enable the degradation of purine alkaloids.