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J Biol Chem. 2015 Jul 24;290(30):18429-37. doi: 10.1074/jbc.M115.663872. Epub 2015 Jun 8.

High Yield Non-detergent Isolation of Photosystem I-Light-harvesting Chlorophyll II Membranes from Spinach Thylakoids: IMPLICATIONS FOR THE ORGANIZATION OF THE PS I ANTENNAE IN HIGHER PLANTS.

Author information

1
From the Department of Biological Sciences, Biochemistry and Molecular Biology Section, Louisiana State University, Baton Rouge, Louisiana 70803.
2
From the Department of Biological Sciences, Biochemistry and Molecular Biology Section, Louisiana State University, Baton Rouge, Louisiana 70803 btbric@lsu.edu.

Abstract

Styrene-maleic acid copolymer was used to effect a non-detergent partial solubilization of thylakoids from spinach. A high density membrane fraction, which was not solubilized by the copolymer, was isolated and was highly enriched in the Photosystem (PS) I-light-harvesting chlorophyll (LHC) II supercomplex and depleted of PS II, the cytochrome b6/f complex, and ATP synthase. The LHC II associated with the supercomplex appeared to be energetically coupled to PS I based on 77 K fluorescence, P700 photooxidation, and PS I electron transport light saturation experiments. The chlorophyll (Chl) a/b ratio of the PS I-LHC II membranes was 3.2 ± 0.9, indicating that on average, three LHC II trimers may associate with each PS I. The implication of these findings within the context of higher plant PS I antenna organization is discussed.

KEYWORDS:

PS I-LHC II supercomplex; electron transfer complex; membrane energetics; membrane protein; photosynthesis; photosystem I; plant biochemistry; stryrene-maleic acid copolymer

PMID:
26055710
PMCID:
PMC4513103
DOI:
10.1074/jbc.M115.663872
[Indexed for MEDLINE]
Free PMC Article

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