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Int Immunopharmacol. 2015 Nov;29(1):81-6. doi: 10.1016/j.intimp.2015.05.011. Epub 2015 May 19.

Unbalanced acetylcholinesterase activity in larynx squamous cell carcinoma.

Author information

1
Molecular Therapy and Biomarkers Research Group, Clinical Analysis Service, University Hospital Virgen de la Arrixaca, IMIB-Arrixaca, Ctra Madrid-Cartagena s/n, El Palmar, 30120 Murcia, Spain; Department of Biochemistry and Molecular Biology A, School of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
2
Otorhinolaryngology Surgical Service, University Hospital Virgen de la Arrixaca IMIB-Arrixaca, Ctra Madrid-Cartagena s/n, El Palmar, 30120 Murcia, Spain.
3
Molecular Therapy and Biomarkers Research Group, Clinical Analysis Service, University Hospital Virgen de la Arrixaca, IMIB-Arrixaca, Ctra Madrid-Cartagena s/n, El Palmar, 30120 Murcia, Spain.
4
Surgery Service of University Hospital Virgen de la Arrixaca IMIB, Ctra Madrid-Cartagena s/n, El Palmar, 30120 Murcia, Spain.
5
Otorhinolaryngology Surgical Service, University Hospital Virgen de la Arrixaca IMIB-Arrixaca, Ctra Madrid-Cartagena s/n, El Palmar, 30120 Murcia, Spain. Electronic address: diego.hellin@um.es.
6
Molecular Therapy and Biomarkers Research Group, Clinical Analysis Service, University Hospital Virgen de la Arrixaca, IMIB-Arrixaca, Ctra Madrid-Cartagena s/n, El Palmar, 30120 Murcia, Spain. Electronic address: juan.cabezas@carm.es.

Abstract

Previous reports have demonstrated that a non-neuronal cholinergic system is expressed aberrantly in airways. A proliferative effect is exerted directly by cholinergic agonists through the activation of nicotinic and muscarinic receptors. In cancer, particularly those related with smoking, the mechanism through which tumour cells respond to aberrantly activated cholinergic signalling is a key question. Fifty paired pieces of larynx squamous cell carcinoma and adjacent non-cancerous tissue were compared in terms of their acetylcholinesterase activity (AChE). The AChE activity in non-cancerous tissues (0.248 ± 0.030 milliunits per milligram of wet tissue; mU/mg) demonstrates that upper respiratory tissues express sufficient AChE activity for controlling the level of acetylcholine (ACh). In larynx carcinomas, the AChE activity decreased to 0.157 ± 0.024 mU/mg (p=0.009). Larynx cancer patients exhibiting low ACh-degrading enzymatic activity had a significantly shorter overall survival (p=0.031). Differences in the mRNA levels of alternatively spliced AChE isoforms and molecular compositions were noted between glottic and supraglottic cancers. Our results suggest that the low AChE activity observed in larynx squamous cell carcinoma may be useful for predicting the outcome of patients.

KEYWORDS:

Cholinergic system; Human airways; Larynx cancer; Non-neuronal compartment

PMID:
26002584
DOI:
10.1016/j.intimp.2015.05.011
[Indexed for MEDLINE]

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