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Mol Cell. 2015 Mar 19;57(6):1099-1109. doi: 10.1016/j.molcel.2015.01.035.

The ubiquitous yybP-ykoY riboswitch is a manganese-responsive regulatory element.

Author information

1
Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892-5430, USA.
2
National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA.
3
Department of Chemistry, University of Wisconsin Oshkosh, Oshkosh, WI 54901, USA.
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Contributed equally

Abstract

The highly structured, cis-encoded RNA elements known as riboswitches modify gene expression upon binding a wide range of molecules. The yybP-ykoY motif was one of the most broadly distributed and numerous bacterial riboswitches for which the cognate ligand was unknown. Using a combination of in vivo reporter and in vitro expression assays, equilibrium dialysis, and northern analysis, we show that the yybP-ykoY motif responds directly to manganese ions in both Escherichia coli and Bacillus subtilis. The identification of the yybP-ykoY motif as a manganese ion sensor suggests that the genes that are preceded by this motif and encode a diverse set of poorly characterized membrane proteins have roles in metal homeostasis.

PMID:
25794618
PMCID:
PMC4376352
DOI:
10.1016/j.molcel.2015.01.035
[Indexed for MEDLINE]
Free PMC Article

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