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Nutr Metab (Lond). 2015 Mar 8;12:7. doi: 10.1186/s12986-015-0002-9. eCollection 2015.

The APOB insertion/deletion polymorphism (rs17240441) influences postprandial lipaemia in healthy adults.

Author information

1
Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading, RG6 6AP UK ; Institute for Cardiovascular and Metabolic Research (ICMR), University of Reading, Reading, UK.
2
Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Reading, RG6 6AP UK ; Department of Nutrition, Norwich Medical School, University of East Anglia, Norwich, UK.
3
Boston Heart Diagnostics, Framingham, MA 01702 USA.
4
Institute for Cardiovascular and Metabolic Research (ICMR), University of Reading, Reading, UK.

Abstract

BACKGROUND:

Apolipoprotein (apo)B is the structural apoprotein of intestinally- and liver- derived lipoproteins and plays an important role in the transport of triacylglycerol (TAG) and cholesterol. Previous studies have examined the association between the APOB insertion/deletion (ins/del) polymorphism (rs17240441) and postprandial lipaemia in response to a single meal; however the findings have been inconsistent with studies often underpowered to detect genotype-lipaemia associations, focused mainly on men, or with limited postprandial characterisation of participants. In the present study, using a novel sequential test meal protocol which more closely mimics habitual eating patterns, we investigated the impact of APOB ins/del polymorphism on postprandial TAG, non-esterified fatty acids, glucose and insulin levels in healthy adults.

FINDINGS:

Healthy participants (n = 147) consumed a standard test breakfast (0 min; 49 g fat) and lunch (330 min; 29 g fat), with blood samples collected before (fasting) and on 11 subsequent occasions until 480 min after the test breakfast. The ins/ins homozygotes had higher fasting total cholesterol, LDL-cholesterol, TAG, insulin and HOMA-IR and lower HDL-cholesterol than del/del homozygotes (P < 0.017). A higher area under the time response curve (AUC) was evident for the postprandial TAG (P < 0.001) and insulin (P = 0.032) responses in the ins/ins homozygotes relative to the del/del homozygotes, where the genotype explained 35% and 7% of the variation in the TAG and insulin AUCs, respectively.

CONCLUSIONS:

In summary, our findings indicate that the APOB ins/del polymorphism is likely to be an important genetic determinant of the large inter-individual variability in the postprandial TAG and insulin responses to dietary fat intake.

KEYWORDS:

APOB gene; Insertion/deletion polymorphism; Postprandial study; Sequential test meals; Signal peptide polymorphism; Triacylglycerol

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