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Diagn Microbiol Infect Dis. 2015 May;82(1):54-6. doi: 10.1016/j.diagmicrobio.2015.01.012. Epub 2015 Jan 31.

Large-scale clinical validation of a lateral flow immunoassay for detection of cryptococcal antigen in serum and cerebrospinal fluid specimens.

Author information

1
Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD.
2
Microbiology Laboratory, The Johns Hopkins Hospital, Baltimore, MD.
3
Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD; Microbiology Laboratory, The Johns Hopkins Hospital, Baltimore, MD.
4
Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD; The Johns Hopkins Bayview Medical Center, Baltimore, MD.
5
Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD; Microbiology Laboratory, The Johns Hopkins Hospital, Baltimore, MD. Electronic address: szhang28@jhmi.edu.

Abstract

We compared a lateral flow immunoassay (LFA) to a currently used enzyme immunoassay for detection of cryptococcal antigen in 396 sera and 651 cerebrospinal fluid specimens. We found 97% concordance between the 2 assays. The LFA detected an additional 22 positives. Overall, the LFA had sensitivity of 100% and specificity of 99.6% for the diagnosis of cryptococcosis. The LFA is rapid, accurate, and easy to perform, and it is suitable for routine patient care testing.

KEYWORDS:

Antigen; Cerebrospinal fluid; Cryptococcus; Lateral flow immunoassay; Serum

[Indexed for MEDLINE]

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