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Plant Cell. 2015 Feb;27(2):463-79. doi: 10.1105/tpc.114.132043. Epub 2015 Feb 13.

Retromer contributes to immunity-associated cell death in Arabidopsis.

Author information

1
Department of Biology, Copenhagen University, Copenhagen 2200, Denmark.
2
Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center of Plant Biology, SE-75007 Uppsala, Sweden.
3
Howard Hughes Medical Institute, Department of Biology, and Carolina Center for Genome Sciences, University of North Carolina, Chapel Hill, North Carolina 27599-3280.
4
Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
5
Department of Biology, Copenhagen University, Copenhagen 2200, Denmark Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center of Plant Biology, SE-75007 Uppsala, Sweden daniel.hofius@slu.se.

Abstract

Membrane trafficking is required during plant immune responses, but its contribution to the hypersensitive response (HR), a form of programmed cell death (PCD) associated with effector-triggered immunity, is not well understood. HR is induced by nucleotide binding-leucine-rich repeat (NB-LRR) immune receptors and can involve vacuole-mediated processes, including autophagy. We previously isolated lazarus (laz) suppressors of autoimmunity-triggered PCD in the Arabidopsis thaliana mutant accelerated cell death11 (acd11) and demonstrated that the cell death phenotype is due to ectopic activation of the LAZ5 NB-LRR. We report here that laz4 is mutated in one of three VACUOLAR PROTEIN SORTING35 (VPS35) genes. We verify that LAZ4/VPS35B is part of the retromer complex, which functions in endosomal protein sorting and vacuolar trafficking. We show that VPS35B acts in an endosomal trafficking pathway and plays a role in LAZ5-dependent acd11 cell death. Furthermore, we find that VPS35 homologs contribute to certain forms of NB-LRR protein-mediated autoimmunity as well as pathogen-triggered HR. Finally, we demonstrate that retromer deficiency causes defects in late endocytic/lytic compartments and impairs autophagy-associated vacuolar processes. Our findings indicate important roles of retromer-mediated trafficking during the HR; these may include endosomal sorting of immune components and targeting of vacuolar cargo.

PMID:
25681156
PMCID:
PMC4456924
DOI:
10.1105/tpc.114.132043
[Indexed for MEDLINE]
Free PMC Article

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