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Elife. 2015 Feb 2;4. doi: 10.7554/eLife.05701.

Kin cell lysis is a danger signal that activates antibacterial pathways of Pseudomonas aeruginosa.

Author information

1
Department of Microbiology, University of Washington, Seattle, United States.
2
Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, United States.
3
Department of Physics, University of Washington, Seattle, United States.

Abstract

The perception and response to cellular death is an important aspect of multicellular eukaryotic life. For example, damage-associated molecular patterns activate an inflammatory cascade that leads to removal of cellular debris and promotion of healing. We demonstrate that lysis of Pseudomonas aeruginosa cells triggers a program in the remaining population that confers fitness in interspecies co-culture. We find that this program, termed P. aeruginosa response to antagonism (PARA), involves rapid deployment of antibacterial factors and is mediated by the Gac/Rsm global regulatory pathway. Type VI secretion, and, unexpectedly, conjugative type IV secretion within competing bacteria, induce P. aeruginosa lysis and activate PARA, thus providing a mechanism for the enhanced capacity of P. aeruginosa to target bacteria that elaborate these factors. Our finding that bacteria sense damaged kin and respond via a widely distributed pathway to mount a complex response raises the possibility that danger sensing is an evolutionarily conserved process.

KEYWORDS:

DAMP; fluorescence microscopy; infectious disease; interbacterial; intercellular signaling; mass spectrometry; microbiology

PMID:
25643398
PMCID:
PMC4348357
DOI:
10.7554/eLife.05701
[Indexed for MEDLINE]
Free PMC Article

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