Format

Send to

Choose Destination
Thromb Res. 2015 Apr;135(4):679-83. doi: 10.1016/j.thromres.2015.01.004. Epub 2015 Jan 9.

Response of platelet concentrates to pressure and temperature changes without impairment of the in vitro function.

Author information

1
Dept. of Anesthesiology, Maastricht University, The Netherlands.
2
Dept. of Anesthesiology, Intensive Care Medicine, Emergency Care Pain Therapy ZOL Genk, Belgium. Electronic address: svanpoucke@gmail.com.
3
Dept. of Anesthesiology, Maastricht University, The Netherlands; Dept. of Anesthesiology, ICU and Perioperative Medicine, HMC, Doha, Qatar.
4
Hematological Laboratory, Maastricht University Medical Centre, Maastricht, the Netherlands.

Abstract

BACKGROUND:

Rapid platelet concentrate (PC) transfusion is crucial for hemostatic resuscitation. Pressure-aided and warmed transfusion combined with pneumatic tube system (PTS) transport from the laboratory to the operating theatre offers a potentially rapid delivery technique. The aim of this study is a quantitative assessment of in vitro platelet function after PTS transport followed by warmed and/or pressure-aided mock transfusions.

METHODS:

Ten PC samples entered a single PTS run and were subsequently aliquoted for testing. PCs were warmed in a blood warmer and/or subjected to pressure-aided mock transfusion at 300 mm Hg on day 2 or day 7 after collection. Platelet function was assessed using light-transmission aggregometry and multiple-electrode aggregometry to measure the response to ADP, arachidonic acid, collagen, and thrombin-receptor activating peptide. Data were analyzed with non-parametric testing; P<0.05 was considered statistically significant.

RESULTS:

Single PTS transport markedly reduced ADP response in fresh PCs. Seven-day storage had a pronounced effect on both ADP and collagen response. All other tested platelet agonists revealed preserved function. Subsequent warming and/or application of pressure did not significantly compromise platelet function.

CONCLUSIONS:

Pressure-aided plus warmed transfusion and PTS transport was not found to be detrimental to the PC. Further clinical studies are required to determine safety and efficacy of the product.

KEYWORDS:

Aggregometry; Platelets; Pneumatic tube system; Transfusion

PMID:
25601171
DOI:
10.1016/j.thromres.2015.01.004
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center