Format

Send to

Choose Destination
J Mol Cell Cardiol. 2015 Jan;78:54-61. doi: 10.1016/j.yjmcc.2014.10.012. Epub 2014 Nov 11.

Mitochondrial protein turnover: methods to measure turnover rates on a large scale.

Author information

1
The NHLBI Proteomics Center at UCLA, Los Angeles, CA 90095, USA; Department of Physiology, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA.
2
The NHLBI Proteomics Center at UCLA, Los Angeles, CA 90095, USA; Department of Physiology, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA; Department of Medicine, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA; Department of Bioinformatics, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA. Electronic address: pping@mednet.ucla.edu.
3
The NHLBI Proteomics Center at UCLA, Los Angeles, CA 90095, USA; Department of Physiology, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA. Electronic address: edwardlau@ucla.edu.

Abstract

Mitochondrial proteins carry out diverse cellular functions including ATP synthesis, ion homeostasis, cell death signaling, and fatty acid metabolism and biogenesis. Compromised mitochondrial quality control is implicated in various human disorders including cardiac diseases. Recently it has emerged that mitochondrial protein turnover can serve as an informative cellular parameter to characterize mitochondrial quality and uncover disease mechanisms. The turnover rate of a mitochondrial protein reflects its homeostasis and dynamics under the quality control systems acting on mitochondria at a particular cell state. This review article summarizes some recent advances and outstanding challenges for measuring the turnover rates of mitochondrial proteins in health and disease. This article is part of a Special Issue entitled "Mitochondria: From Basic Mitochondrial Biology to Cardiovascular Disease".

KEYWORDS:

Heavy water; Mitochondria; Protein turnover; Proteome dynamics; Proteomics

PMID:
25451168
PMCID:
PMC4746024
DOI:
10.1016/j.yjmcc.2014.10.012
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center