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J Biomed Opt. 2014;19(10):108003. doi: 10.1117/1.JBO.19.10.108003.

Transcranial low-level laser therapy enhances learning, memory, and neuroprogenitor cells after traumatic brain injury in mice.

Author information

1
Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Department of Otolaryngology, Nanning 530021, ChinabWellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114, United StatescHarvard Medical School.
2
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114, United StatescHarvard Medical School, Department of Dermatology, Boston, Massachusetts 02115, United States.
3
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114, United StatescHarvard Medical School, Department of Dermatology, Boston, Massachusetts 02115, United StatesdGuangxi Medical University, First Affiliated College.
4
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114, United StatescHarvard Medical School, Department of Dermatology, Boston, Massachusetts 02115, United StateseHarvard-MIT Division of Health Sciences and Technolo.

Abstract

The use of transcranial low-level laser (light) therapy (tLLLT) to treat stroke and traumatic brain injury (TBI) is attracting increasing attention. We previously showed that LLLT using an 810-nm laser 4 h after controlled cortical impact (CCI)-TBI in mice could significantly improve the neurological severity score, decrease lesion volume, and reduce Fluoro-Jade staining for degenerating neurons. We obtained some evidence for neurogenesis in the region of the lesion. We now tested the hypothesis that tLLLT can improve performance on the Morris water maze (MWM, learning, and memory) and increase neurogenesis in the hippocampus and subventricular zone (SVZ) after CCI-TBI in mice. One and (to a greater extent) three daily laser treatments commencing 4-h post-TBI improved neurological performance as measured by wire grip and motion test especially at 3 and 4 weeks post-TBI. Improvements in visible and hidden platform latency and probe tests in MWM were seen at 4 weeks. Caspase-3 expression was lower in the lesion region at 4 days post-TBI. Double-stained BrdU-NeuN (neuroprogenitor cells) was increased in the dentate gyrus and SVZ. Increases in double-cortin (DCX) and TUJ-1 were also seen. Our study results suggest that tLLLT may improve TBI both by reducing cell death in the lesion and by stimulating neurogenesis.

PMID:
25292167
PMCID:
PMC4189010
DOI:
10.1117/1.JBO.19.10.108003
[Indexed for MEDLINE]
Free PMC Article

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