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Cold Spring Harb Protoc. 2014 Oct 1;2014(10):pdb.top074542. doi: 10.1101/pdb.top074542.

Isolation of mitochondria from cells and tissues.

Author information

1
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147-2408;
2
Department of Pathology, Pathology Research, Yale University School of Medicine, New Haven, Connecticut 06520-8023; Department of Genetics, Pathology Research, Yale University School of Medicine, New Haven, Connecticut 06520-8023.

Abstract

Mitochondria are complex organelles at the center of cellular metabolism, apoptosis, and signaling. They continue to be the subject of intense basic investigation to understand their composition and function, but they have also captivated the attention of clinical researchers because of the growing knowledge of the (sometimes unexpected) roles of mitochondria in human diseases and aging. A full understanding of these intriguing organelles often requires their purification from cells or tissues under specific physiological or pathological conditions. Here we provide some introductory considerations for those interested in purifying mitochondria for subsequent downstream biophysical, structural, and functional analysis.

PMID:
25275115
DOI:
10.1101/pdb.top074542
[Indexed for MEDLINE]

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